7UX5
Structure of PDL1 in complex with FP28136, a Helicon Polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-11 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.99984 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.477, 77.882, 127.747 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.020 - 3.350 |
R-factor | 0.2365 |
Rwork | 0.233 |
R-free | 0.30070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c3t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.046 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4444) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.020 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.199 | 0.750 |
Number of reflections | 11440 | 25562 |
<I/σ(I)> | 9.9 | |
Completeness [%] | 99.6 | |
Redundancy | 10.8 | |
CC(1/2) | 0.996 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M TRIS pH 8, 25% v/v PEG 350 MME. |