7UX0

Human Sperm TMEM95 Ectodomain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	39.530, 43.113, 72.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 1.490
R-factor	0.2238
Rwork	0.223
R-free	0.25050
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.010	1.520
High resolution limit [Å]	1.490	1.490
Number of reflections	20505	1004
<I/σ(I)>	12.3
Completeness [%]	99.7
Redundancy	12.6
CC(1/2)	0.997	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	150 mM NaCl, 20 mM HEPES pH 7.3, 30 mM CaCl2, 2% (w/v) PPG-P400, and 22% (w/v) PEG 3,350