7UWW
Sas6 with alpha-cyclodextrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.450, 82.530, 213.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.995 - 1.610 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7uwu |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.650 |
| High resolution limit [Å] | 1.610 | 7.200 | 1.610 |
| Rmerge | 0.092 | 0.060 | 1.799 |
| Rmeas | 0.095 | 0.063 | 1.889 |
| Rpim | 0.025 | 0.017 | 0.563 |
| Total number of observations | 1690626 | 25810 | 29309 |
| Number of reflections | 122358 | 1956 | 2727 |
| <I/σ(I)> | 16 | 36.3 | 1.1 |
| Completeness [%] | 77.0 | 98.6 | 23.5 |
| Redundancy | 13.8 | 13.2 | 10.7 |
| CC(1/2) | 0.999 | 0.998 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 293 | PEG 3350, Potassium thiocyanate, TEW |






