7UWU
Starch adherence system protein 6 (Sas6)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.170, 82.370, 213.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.770 - 2.190 |
R-factor | 0.1893 |
Rwork | 0.186 |
R-free | 0.25510 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.851 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.770 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.090 | 0.190 |
Number of reflections | 63244 | 309 |
<I/σ(I)> | 22.7 | 14.3 |
Completeness [%] | 99.3 | |
Redundancy | 16.6 | |
CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, potassium thiocyanate, Anderson Evans polyoxotungstate (TEW) |