7UW8
Drosophila melanogaster setdb1-tuor domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-03 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54178 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 59.989, 110.673, 115.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.910 - 2.500 |
R-factor | 0.238 |
Rwork | 0.237 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bhd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.920 | 39.920 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.080 | 0.019 | 1.369 |
Rmeas | 0.086 | 0.021 | 1.474 |
Rpim | 0.032 | 0.008 | 0.541 |
Total number of observations | 96242 | 2033 | 10344 |
Number of reflections | 13531 | 331 | 1451 |
<I/σ(I)> | 21.7 | 83.5 | 1.9 |
Completeness [%] | 99.4 | 98.2 | 97 |
Redundancy | 7.1 | 6.1 | 7.1 |
CC(1/2) | 0.999 | 0.999 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 1.6M ammonium sulphate, 0.01M Magnesium Chloride,0.1M tris pH8.5 |