7UW3

Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-30
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	58.943, 93.066, 96.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.410 - 1.700
R-factor	0.2834
Rwork	0.283
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3m
RMSD bond length	0.003
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.410	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.097
Rmeas	0.100
Rpim	0.039
Number of reflections	57729	3009
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	13.5
CC(1/2)	0.990	0.210

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	30% PEG4000, 100 mM MES, pH 6.5