7UVR

Crystal structure of human ClpP protease in complex with TR-65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9201
Spacegroup name	C 1 2 1
Unit cell lengths	142.171, 152.515, 104.268
Unit cell angles	90.00, 118.10, 90.00

Refinement procedure

Resolution	29.350 - 2.860
R-factor	0.2052
Rwork	0.203
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bba
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.350	2.930
High resolution limit [Å]	2.860	2.860
Rmerge	0.207
Number of reflections	44929	44929
<I/σ(I)>	6
Completeness [%]	99.2
Redundancy	5.2
CC(1/2)	0.987	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M sodium acetate pH 4.6 to 5.2, 5% PEG 4000