7UVG
First cohesin of Sca5 from Ruminococcus bromii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | .979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 31.040, 137.790, 85.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 - 1.770 |
| R-factor | 0.2211 |
| Rwork | 0.213 |
| R-free | 0.27720 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.182 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.040 | 22.000 | 1.820 |
| High resolution limit [Å] | 1.770 | 7.920 | 1.770 |
| Rmerge | 0.051 | 0.032 | 0.997 |
| Number of reflections | 17738 | 15989 | 1749 |
| <I/σ(I)> | 11.47 | ||
| Completeness [%] | 96.4 | 93.7 | 96.5 |
| Redundancy | 3.8 | 3.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ZnCl2, 18% PEG 8000, 100mM Na cacodylate |
