7UUN
Crystal structure of aminoglycoside resistance enzyme ApmA, complex with neomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 133.564, 133.564, 133.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.800 - 2.830 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jm2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.880 |
High resolution limit [Å] | 2.830 | 2.830 |
Rmerge | 0.102 | 4.877 |
Rpim | 0.016 | 0.770 |
Number of reflections | 10203 | 492 |
<I/σ(I)> | 54.3 | 1.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 40.9 | |
CC(1/2) | 1.000 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 25% PEG3350, 10 mM neomycin |