7UU5

Crystal structure of APOBEC3G complex with 5'-Overhang dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03322
Spacegroup name	P 1
Unit cell lengths	61.305, 71.559, 72.710
Unit cell angles	119.39, 111.00, 90.08

Refinement procedure

Resolution	46.508 - 2.900
R-factor	0.1907
Rwork	0.187
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	21497	2116
<I/σ(I)>	11.56
Completeness [%]	98.2
Redundancy	3.3
CC(1/2)	0.994	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.8 M Na/K phosphate, pH 6.9