7UU4

Crystal structure of APOBEC3G complex with ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03318
Spacegroup name	P 21 21 21
Unit cell lengths	54.225, 67.976, 126.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.358 - 2.100
R-factor	0.1853
Rwork	0.184
R-free	0.20410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	51798	2400
<I/σ(I)>	14.9
Completeness [%]	97.9
Redundancy	12.9
CC(1/2)	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.22 M Na2SO4, 20% PEG 3350