7UTJ
Cryogenic electron microscopy 3D map of F-actin bound by human dimeric alpha-catenin
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Phase Plate | OTHER |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.77 |
| Software | PHENIX,cryoSPARC,SerialEM,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
