7UTG

2D9 nanobody to BCL11A-exZF23 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	36.619, 52.247, 54.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.440 - 1.250
R-factor	0.1951
Rwork	0.193
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nlb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.440	1.270
High resolution limit [Å]	1.250	1.250
Number of reflections	54642	1476
<I/σ(I)>	1.82
Completeness [%]	97.3	75
Redundancy	7.4
CC(1/2)	0.834	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5