7URP

Second cohesin module from Sca5 of Ruminococcus bromii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-10
Detector	RAYONIX MX300-HS
Wavelength(s)	.729
Spacegroup name	C 1 2 1
Unit cell lengths	120.570, 30.050, 49.940
Unit cell angles	90.00, 94.53, 90.00

Refinement procedure

Resolution	30.070 - 1.030
R-factor	0.1436
Rwork	0.142
R-free	0.16470
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.511
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.100	30.050	1.060
High resolution limit [Å]	1.030	4.610	1.030
Rmerge	0.080	0.056
Number of reflections	87298	1028	6377
<I/σ(I)>	12
Completeness [%]	98.6	95.4	98.3
Redundancy	13.4	12.7	8.7
CC(1/2)			0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.06M CaCl2, 0.06M MgCl2, 0.1M imidazole, 0.1M MES pH = 6.5, 30% PEG 20K, 30% PEGMME 500