7UQV
Pseudobacteroides cellulosolvens pseudo-CphB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.522 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 172.797, 172.797, 116.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 92.140 - 2.400 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3en0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2-4158_final) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 149.650 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 76986 | 6145 |
<I/σ(I)> | 14.96 | |
Completeness [%] | 97.8 | |
Redundancy | 18.1 | |
CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.3 M magnesium formate |