7UQU

Crystal Structure of Mouse Cadherin-23 EC9

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	49.308, 41.661, 56.908
Unit cell angles	90.00, 114.20, 90.00

Refinement procedure

Resolution	51.909 - 1.976
Rwork	0.180
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tfl 5i8d
RMSD bond length	0.008
RMSD bond angle	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.909	2.030
High resolution limit [Å]	1.976	2.000
Number of reflections	14817	701
<I/σ(I)>	13.4	4.8
Completeness [%]	97.0
Redundancy	4.2
CC(1/2)	0.991	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Magnesium Chloride 20% PEG 3350