7UQC

phospho-GlialCAM peptide AA370-389 with Fab MS39p2w174

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	195.027, 99.514, 136.414
Unit cell angles	90.00, 112.81, 90.00

Refinement procedure

Resolution	44.110 - 2.650
R-factor	0.1788
Rwork	0.176
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7k7r
RMSD bond length	0.010
RMSD bond angle	1.279
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.110	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.113	1.461
Rmeas	0.160
Rpim	0.113	0.940
Number of reflections	69508	10053
<I/σ(I)>	5.42	1.1
Completeness [%]	98.4	97.6
Redundancy	3.5	3.4
CC(1/2)	0.977	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	tbd