7UQA
Crystal structure of the small Ultra-Red Fluorescent Protein (smURFP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.087, 141.802, 171.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.572 - 2.802 |
R-factor | 0.2406 |
Rwork | 0.239 |
R-free | 0.26340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4PO5 CHAIN E |
RMSD bond length | 0.003 |
RMSD bond angle | 0.619 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.140 | 1.190 |
Number of reflections | 24645 | 2435 |
<I/σ(I)> | 9.99 | 1.25 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 3.9 | 3.6 |
CC(1/2) | 0.994 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4M NaNO3, 33.3mM NaCH3COO.3H2O (pH 4.6) |