7UPS
Structural study of Legionella pneumophila effector DotY (Lpg0294)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97935 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.200, 75.620, 101.200 |
Unit cell angles | 90.00, 127.91, 90.00 |
Refinement procedure
Resolution | 43.540 - 2.430 |
R-factor | 0.2379 |
Rwork | 0.237 |
R-free | 0.26220 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.567 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.550 | 2.550 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 33201 | 3535 |
<I/σ(I)> | 9.35 | |
Completeness [%] | 95.5 | |
Redundancy | 6.4 | |
CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Sodium Citrate pH3.5, 25% PEG8k and 0.3M Sodium Acetate |