7UPJ
HIV-1 PROTEASE/U101935 COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-09-22 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.374, 89.605, 47.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EARLIER STRUCTURE FROM SAME LABORATORY |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.624 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | MERLOT |
| Refinement software | CEDAR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.243 |
| Number of reflections | 13817 | |
| <I/σ(I)> | 14 | 2.9 |
| Completeness [%] | 75.8 | 31 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | inhibitor | 0.1 (mg/ml) | |
| 2 | 1 | drop | protease | 6 (mg/ml) | |
| 3 | 1 | reservoir | acetate | 0.1 (M) | precipitant |
| 4 | 1 | reservoir | citrate | 0.1 (M) | precipitant |
| 5 | 1 | reservoir | sodium chloride | precipitant | |
| 6 | 1 | drop | precipitant |
