7UON
CTX-M-14 Y105W mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00001 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 41.430, 41.430, 230.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.320 - 1.350 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.848 |
Data reduction software | iMOSFLM (7.4.0) |
Data scaling software | pointless (1.11.17) |
Phasing software | PHASER (2.1.2) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.320 | 1.420 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.066 | 0.553 |
Rmeas | 0.069 | 0.580 |
Rpim | 0.173 | |
Number of reflections | 51539 | 7337 |
<I/σ(I)> | 17 | 3.4 |
Completeness [%] | 99.1 | 98.1 |
Redundancy | 12.3 | 10.8 |
CC(1/2) | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | HEPES (0.1M), PEG 4,000 (25% w/v) |