7UOI
Crystallographic structure of DapE from Enterococcus faecium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.85 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.486, 45.378, 78.751 |
| Unit cell angles | 90.00, 103.65, 90.00 |
Refinement procedure
| Resolution | 38.263 - 1.600 |
| R-factor | 0.1542 |
| Rwork | 0.153 |
| R-free | 0.18070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.420 |
| Number of reflections | 58807 | 7861 |
| <I/σ(I)> | 11.2 | 2.9 |
| Completeness [%] | 97.7 | 90 |
| Redundancy | 5.5 | 4.2 |
| CC(1/2) | 0.960 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 100 mM sodium acetate, 8% PEG4000, pH 4.6 |
