7UN7
DNA Polymerase lambda in complex with a 1nt microhomology substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.135, 62.774, 140.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.760 - 2.040 |
R-factor | 0.2212 |
Rwork | 0.220 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3upq |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.113 | 52.113 | 2.089 |
High resolution limit [Å] | 2.040 | 5.845 | 2.040 |
Rmerge | 0.200 | 0.072 | 9.900 |
Rmeas | 0.385 | 0.075 | 10.876 |
Rpim | 0.106 | 0.022 | 2.986 |
Number of reflections | 30399 | 1519 | 1522 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 97.3 | 100 | 98.6 |
Redundancy | 13.3 | 12.9 | 13.8 |
CC(1/2) | 0.932 | 0.901 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG |