7UMJ
Crystal structure of recombinant Solieria filiformis lectin (rSfL)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.054, 62.430, 90.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.350 - 1.880 |
| R-factor | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gu8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.805 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.350 | 1.940 |
| High resolution limit [Å] | 1.460 | 1.880 |
| Number of reflections | 80687 | 22337 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 87.9 | 95.77 |
| Redundancy | 6.5 | 2 |
| CC(1/2) | 0.997 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES pH 7.5, 100 mM sodium chloride, 1.6 M ammonium sulfate |
