7UMI
Importin a1 bound to Cp183-CTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.436, 90.688, 97.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.090 - 1.990 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7leu |
RMSD bond length | 0.008 |
RMSD bond angle | 0.945 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 1.990 | 1.990 |
Rpim | 0.033 | 0.605 |
Number of reflections | 47594 | 47594 |
<I/σ(I)> | 11.5 | 1.5 |
Completeness [%] | 98.8 | 99.3 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.6 M sodium citrate, 0.1 M HEPES pH 7.2, 10 mM DTT |