7ULQ

Recombinant Hannalgesin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999943
Spacegroup name	P 32
Unit cell lengths	65.509, 65.509, 164.752
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.720 - 2.200
R-factor	0.2131
Rwork	0.211
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ntn 6zfm
RMSD bond length	0.007
RMSD bond angle	1.003
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.730	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.089	0.883
Rmeas	0.108
Rpim	0.061	0.611
Number of reflections	46141	3741
<I/σ(I)>	10.5	2.2
Completeness [%]	99.9	100
Redundancy	6.1
CC(1/2)		0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	rec-hannalgesin at 80 mg/ml in 200 mM NH4Ac (pH 7.0), 1:1 with 0.1 M Bis-Tris, 0.2 M (NH4)2SO4, 25% PEG3350, at 18 degree centigrade