7ULO
Potato leafroll virus N-terminal readthrough domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 63.229, 65.147, 109.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.010 - 2.210 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23920 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.150 | 65.150 | 2.290 |
| High resolution limit [Å] | 2.210 | 8.850 | 2.210 |
| Rmerge | 0.097 | 0.069 | 0.850 |
| Rmeas | 0.107 | 0.076 | 1.103 |
| Rpim | 0.045 | 0.031 | 0.693 |
| Total number of observations | 115339 | 2022 | 3184 |
| Number of reflections | 22427 | 403 | 1558 |
| <I/σ(I)> | 10.5 | 27.4 | 0.8 |
| Completeness [%] | 96.8 | 95.3 | 75.2 |
| Redundancy | 5.1 | 5 | 2 |
| CC(1/2) | 0.996 | 0.997 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1 M Tris-HCl, pH 8.5, 0.25 M ammonium sulfate, 14% PEG8000, 3% ethylene glycol, 5 mM DTT |
