7ULN
Turnip yellows virus N-terminal readthrough domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.434, 74.859, 130.779 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.389 - 1.530 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.19640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ulo |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 130.780 | 130.780 | 1.550 |
High resolution limit [Å] | 1.530 | 8.360 | 1.530 |
Rmerge | 0.070 | 0.041 | 0.729 |
Rmeas | 0.076 | 0.045 | 0.794 |
Rpim | 0.029 | 0.017 | 0.310 |
Total number of observations | 3062 | 20257 | |
Number of reflections | 69780 | 514 | 3206 |
<I/σ(I)> | 20.5 | 62.3 | 2.2 |
Completeness [%] | 99.5 | 99.1 | 93.1 |
Redundancy | 6.7 | 6 | 6.3 |
CC(1/2) | 0.999 | 0.999 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293.15 | 0.1 M HEPES, pH 7.7, 1.4 M ammonium sulfate, 0.1 M sodium chloride |