7ULD
GTP complex of F420-gamma glutamyl ligase (CofE) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95370 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 68.544, 68.544, 93.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.040 - 1.300 |
R-factor | 0.157 |
Rwork | 0.156 |
R-free | 0.18210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2phn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.374 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rpim | 0.031 | 0.693 |
Number of reflections | 55332 | 2692 |
<I/σ(I)> | 12.3 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 28 | 27.2 |
CC(1/2) | 0.999 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8 M ammonium sulfate, 0.1 M citrate pH 4.5, 2 mM GTP, 5 mM Mn2+ |