7ULA
Structure of the Pseudomonas putida AlgKX modification and secretion complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.512 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 169.950, 169.950, 143.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.548 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.063 | 1.158 |
Number of reflections | 38142 | 3741 |
<I/σ(I)> | 33.2 | |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 13.9 | 9 |
CC(1/2) | 1.000 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.1 M Tris-HCl pH = 8.5, 0.01 M Nickel (II) chloride, 20% (w/v) PEG2000 MME |