7UKO

Integrin alpha IIB beta3 complex with sibrafiban (Mn)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	258.388, 144.599, 104.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.287 - 2.604
R-factor	0.2145
Rwork	0.214
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t3p
RMSD bond length	0.007
RMSD bond angle	0.603
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	135279	9924
<I/σ(I)>	6.74
Completeness [%]	99.3
Redundancy	4.6
CC(1/2)	0.233	0.110

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	277	11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl