7UKN

Crystal Structure of DDB1 in Complex with the H-Box Motif of pUL145

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.535, 134.664, 182.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.210 - 2.900
R-factor	0.1976
Rwork	0.194
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i7l
RMSD bond length	0.009
RMSD bond angle	1.219
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.210	2.920
High resolution limit [Å]	2.870	2.870
Rmerge	0.132	1.793
Rmeas	0.180
Rpim	0.070	0.840
Number of reflections	34718	2877
<I/σ(I)>	12.7	1.37
Completeness [%]	97.4	82.67
Redundancy	6.3	6.6
CC(1/2)	0.992	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES (pH 6.5-6.7), 16% PEG 4000, 50 mM NaCl, 5 mM DTT