7UK3
Crystal structure of queuine salvage enzyme DUF2419, wild-type (non-His6x tagged)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.159, 95.159, 129.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.240 - 2.310 |
| R-factor | 0.1518 |
| Rwork | 0.150 |
| R-free | 0.17460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7u07 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-3000 (v721.3) |
| Data scaling software | HKL-3000 (v721.3) |
| Phasing software | PHENIX (v1.19.2) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.000 | 42.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.265 | 0.067 | 6.599 |
| Rmeas | 0.279 | 0.070 | 6.971 |
| Rpim | 0.086 | 0.022 | 2.225 |
| Total number of observations | 312644 | ||
| Number of reflections | 30413 | 1660 | 1479 |
| <I/σ(I)> | 3.6 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 10.3 | 9.9 | 9.6 |
| CC(1/2) | 0.998 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M Sodium thiocynate, 20% PEG3350, pH 6.9 |
