7UJK
Integrin alaphIIBbeta3 complex with lamifiban
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 259.379, 145.247, 105.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.778 - 2.433 |
R-factor | 0.221446528373 |
Rwork | 0.221 |
R-free | 0.24744 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t3p |
RMSD bond length | 0.005 |
RMSD bond angle | 0.561 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 |
High resolution limit [Å] | 2.433 | 2.450 |
Number of reflections | 145100 | 10651 |
<I/σ(I)> | 8 | |
Completeness [%] | 99.2 | |
Redundancy | 3.9 | |
CC(1/2) | 0.992 | 0.344 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 277 | 11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl |