7UJI

Structure of the P130R single variant of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-10-22
Detector	RDI CMOS_8M
Wavelength(s)	1.000003
Spacegroup name	P 2 21 21
Unit cell lengths	55.742, 127.006, 129.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.470 - 2.300
R-factor	0.1981
Rwork	0.195
R-free	0.25920
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1uxh
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.158
Rpim	0.059	0.964
Number of reflections	41612	27361
<I/σ(I)>	13.3	0.8
Completeness [%]	99.7	99.9
Redundancy	7.1	6.7
CC(1/2)	0.997	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 - 0.25 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21-23% (w/v) PEG MME 2000