7UJI
Structure of the P130R single variant of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2019-10-22 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000003 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.742, 127.006, 129.239 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.470 - 2.300 |
R-factor | 0.1981 |
Rwork | 0.195 |
R-free | 0.25920 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1uxh |
RMSD bond length | 0.008 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.470 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.158 | |
Rpim | 0.059 | 0.964 |
Number of reflections | 41612 | 27361 |
<I/σ(I)> | 13.3 | 0.8 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 7.1 | 6.7 |
CC(1/2) | 0.997 | 0.469 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8 - 0.25 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21-23% (w/v) PEG MME 2000 |