7UJH

Structure of the P130R single variant of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP and glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	P 2 21 21
Unit cell lengths	55.851, 126.036, 129.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.020 - 2.240
R-factor	0.206
Rwork	0.204
R-free	0.24800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1uxi
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.040	2.310
High resolution limit [Å]	2.240	2.240
Rmerge	0.129	2.420
Rmeas	0.139	2.603
Rpim	0.052	0.954
Number of reflections	44709	4053
<I/σ(I)>	9.1	0.9
Completeness [%]	100.0	100
Redundancy	7.2	7.2
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 - 0.25 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21-23% (w/v) PEG MME 2000