7UJH
Structure of the P130R single variant of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP and glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.851, 126.036, 129.606 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.020 - 2.240 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.24800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1uxi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.946 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.040 | 2.310 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.129 | 2.420 |
Rmeas | 0.139 | 2.603 |
Rpim | 0.052 | 0.954 |
Number of reflections | 44709 | 4053 |
<I/σ(I)> | 9.1 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.2 |
CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8 - 0.25 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21-23% (w/v) PEG MME 2000 |