7UJ3

Crystal structure of Human respiratory syncytial virus F variant (construct pXCS847A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 41 3 2
Unit cell lengths	170.020, 170.020, 170.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.080 - 3.500
R-factor	0.2374
Rwork	0.236
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmu
RMSD bond length	0.008
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	BUSTER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	3.710
High resolution limit [Å]	3.500	3.500
Number of reflections	11179	1742
<I/σ(I)>	28.2
Completeness [%]	99.8
Redundancy	38.3
CC(1/2)	1.000	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Na acetate, pH 4.6, 0.2 M ammonium sulfate, and 30% poly(ethylene) glycol monomethyl ether 2000