7UIE
Crystal structure of HcE-JLE-G6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 88.737, 174.626, 214.439 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 135.410 - 3.230 |
R-factor | 0.2318 |
Rwork | 0.230 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 174.630 | 3.320 |
High resolution limit [Å] | 3.230 | 3.230 |
Rpim | 0.106 | 0.874 |
Number of reflections | 54051 | 5309 |
<I/σ(I)> | 8.6 | 1.06 |
Completeness [%] | 99.6 | |
Redundancy | 4.1 | |
CC(1/2) | 0.987 | 0.328 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES, pH 7.0, 0.2 M NaCl and 18% PEG 3350 |