7UGT

Crystal structure of hyperfolder fluorescent protein FOLD6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 64
Unit cell lengths	61.438, 61.438, 112.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.632 - 1.210
R-factor	0.1465
Rwork	0.146
R-free	0.17100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wj2
RMSD bond length	0.004
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.632	1.253
High resolution limit [Å]	1.210	1.210
Rmerge	0.037	1.128
Rmeas	0.041	1.552
Rpim	0.017	1.060
Number of reflections	117807	1788
<I/σ(I)>	18.36	0.36
Completeness [%]	87.9	24.56
Redundancy	4.8	1.2
CC(1/2)	1.000	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Tris-HCl pH 8.5, 30% PEG 4000, 100 mM magnesium chloride