7UGR
Crystal structure of hyperfolder YFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.994, 66.872, 143.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.393 - 1.740 |
R-factor | 0.2033 |
Rwork | 0.202 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wj2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.393 | 1.802 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.085 | 0.325 |
Rmeas | 0.103 | 0.392 |
Rpim | 0.057 | 0.216 |
Number of reflections | 46199 | 2564 |
<I/σ(I)> | 8.58 | 2.46 |
Completeness [%] | 97.4 | 99.77 |
Redundancy | 3.1 | 3.2 |
CC(1/2) | 0.989 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M Tris-HCl pH 8.5, 25% PEG 3350, 0.2 M sodium acetate |