7UG8
Crystal structure of a solute receptor from Synechococcus CC9311 in complex with alpha-ketovaleric and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 50 |
| Detector technology | PIXEL |
| Collection date | 2019-12-12 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552, 3.1790, 3.4925, 4.7686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.680, 54.750, 61.150 |
| Unit cell angles | 69.65, 77.73, 67.27 |
Refinement procedure
| Resolution | 50.277 - 1.796 |
| Rwork | 0.169 |
| R-free | 0.18980 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.277 | 50.230 |
| High resolution limit [Å] | 1.796 | 9.060 |
| Rmerge | 0.068 | 0.039 |
| Rmeas | 0.073 | 0.041 |
| Rpim | 0.024 | 0.012 |
| Number of reflections | 50762 | 451 |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 90.9 | |
| Redundancy | 13.6 | 23.3 |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.02 M Sodium/Potassium Phosphate, 0.1M Bis Tris propane 7.5, 20% Peg 3350 Cryo: 30 % Peg 400 |
