7UFW
Pfs230 D1D2 in complex with LMIV230-01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.440, 151.370, 99.040 |
Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.100 |
R-factor | 0.1911 |
Rwork | 0.190 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jum |
RMSD bond length | 0.003 |
RMSD bond angle | 0.672 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.920 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 75653 | 5372 |
<I/σ(I)> | 11 | |
Completeness [%] | 99.5 | |
Redundancy | 9.6 | |
CC(1/2) | 0.990 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Hepes, pH 6.5, 1.0 M lithium chloride, 20% PEG 6000 |