7UFV
Crystal structure of the WDR domain of human DCAF1 in complex with OICR-6766
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-24 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.140, 87.875, 73.807 |
| Unit cell angles | 90.00, 98.54, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7sse |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.980 | 43.940 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.062 | 0.046 | 0.923 |
| Rmeas | 0.072 | 0.053 | 1.070 |
| Rpim | 0.036 | 0.026 | 0.534 |
| Total number of observations | 1722 | 11897 | |
| Number of reflections | 48379 | 460 | 3107 |
| <I/σ(I)> | 9.6 | 25.1 | 1.5 |
| Completeness [%] | 99.0 | 97.5 | 99.5 |
| Redundancy | 3.8 | 3.7 | 3.8 |
| CC(1/2) | 0.998 | 0.997 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 20% PEG3350, 0.2M Potassium Fluoride |
