7UFQ
Structure of PfCSP peptide 21 with antibody P3-43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.482, 61.697, 89.604 |
Unit cell angles | 90.00, 103.74, 90.00 |
Refinement procedure
Resolution | 43.520 - 2.300 |
R-factor | 0.2189 |
Rwork | 0.215 |
R-free | 0.28480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b5m |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.520 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 34697 | 3381 |
<I/σ(I)> | 8.92 | |
Completeness [%] | 97.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.984 | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris hydrochloride pH 8.5, 2.25 M Ammonium hydrogen phosphate |