7UFQ

Structure of PfCSP peptide 21 with antibody P3-43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	74.482, 61.697, 89.604
Unit cell angles	90.00, 103.74, 90.00

Refinement procedure

Resolution	43.520 - 2.300
R-factor	0.2189
Rwork	0.215
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5m
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.520	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	34697	3381
<I/σ(I)>	8.92
Completeness [%]	97.5
Redundancy	3.4
CC(1/2)	0.984	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris hydrochloride pH 8.5, 2.25 M Ammonium hydrogen phosphate