7UFO

Structure of PfCSP peptide 21 with antibody P3-42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	94.505, 60.691, 86.502
Unit cell angles	90.00, 106.18, 90.00

Refinement procedure

Resolution	46.430 - 1.800
R-factor	0.1932
Rwork	0.192
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5m
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.430	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	42986	4118
<I/σ(I)>	7.51
Completeness [%]	98.2
Redundancy	3.5
CC(1/2)	0.988	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris hydrochloride pH 8.5, 2.8 M ammonium sulfate