7UFN
Structure of PfCSP peptide 21 with antibody P3-21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.694, 124.033, 103.635 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 51.820 - 2.200 |
R-factor | 0.1662 |
Rwork | 0.164 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b5m |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.820 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 46287 | 4700 |
<I/σ(I)> | 5.74 | |
Completeness [%] | 99.8 | |
Redundancy | 3.6 | |
CC(1/2) | 0.980 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 5% isopropanol, 2 M Lithium sulfate, 0.1 M Magnesium sulfate, and 0.1 M sodium acetate trihydrate pH 4.5 |