7UF2

RibB from Vibrio cholera bound with D-xylulose-5-phosphate (D-Xy5P) and manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	61.029, 79.106, 91.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 2.000
R-factor	0.1695
Rwork	0.164
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p8e
RMSD bond length	0.011
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.043	0.264
Number of reflections	15176	1092
<I/σ(I)>	16.7	4.2
Completeness [%]	99.3
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M lithium acetate, 12-18% w/v PEG3350