7UEZ
Apo RibB from Vibrio cholera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8526 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.837, 73.897, 95.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.950 - 1.080 |
| R-factor | 0.1348 |
| Rwork | 0.135 |
| R-free | 0.15130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p8j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.950 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rpim | 0.038 | 0.401 |
| Number of reflections | 172713 | 8395 |
| <I/σ(I)> | 13 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 298 | 0.1 M sodium phosphate, pH 9.3, 16% w/v PEG3350, 0.3 M glycine |
