7UEH

Pyruvate kinase from Zymomonas mobilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	207.929, 256.126, 82.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.330 - 2.400
R-factor	0.1882
Rwork	0.185
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e28
RMSD bond length	0.013
RMSD bond angle	1.269
Data reduction software	autoPROC
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.400	2.442
High resolution limit [Å]	2.400	2.400
Rpim	0.065	0.382
Number of reflections	170665	8504
<I/σ(I)>	2.1
Completeness [%]	98.7	99
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	65 mM potassium thiocyanate, 29.5% PEG3350