7UDO
Crystal structure of designed helical repeat protein RPB_LRP2_R4 (proteolysis fragment?), forming pseudopolymeric filaments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 72.620, 72.620, 127.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.730 - 2.500 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | predicted model |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.730 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.070 | 2.180 |
| Number of reflections | 13974 | 1002 |
| <I/σ(I)> | 18.6 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.8 |
| CC(1/2) | 1.000 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M potassium phosphate dibasic, 20% w/v PEG3350 |
